研發出往復式熱循環反應器（Oscillating thermocycler），樣本置放於一個鋁製反應槽，此反應槽固定於線性馬達的移動平台上，而在系統建立三個固定的溫度區，樣本的升降溫則藉由線性馬達的往復式移動，使反應物在一個直線通道上，接觸不同的溫度區，達到反轉錄聚合酶連鎖反應（Reverse Transcription Polymerase Chain Reaction，RT-PCR）的反應溫度和反應時間。裂解區放置在黏合區和延伸區之間，裝置可進行canine distemper virus的RNA檢測。

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The reverse transcription-polymerase chain reaction (RT-PCR) has been utilized as an effective tool to diagnose the infectious diseases of viruses. In the present work, the oscillating thermocycler is fabricated and performed to successfully carry out the one-step RT-PCR process.

The ribonucleic acid (RNA) mixture is pipetted into the fixed sample volume inside an aluminum reaction block. The sample oscillates the pathway onto the linear motion control system and through the specific RT-PCR heating zones with individual homemade thermal control modules. The oscillating thermocycler combines the merits of the chamber type and the CF type systems.

Before PCR, the reaction chamber moves to the low-temperature zone to complete the RT stage and synthesize the complementary deoxyribonucleic acid (DNA). Next, the low-temperature zone is regulated to the annealing zone. Furthermore, the reactive sample is moved back and forth among three isothermal zones to complete PCR. No extra heating zone is required for the RT stage. The total length of the moving displacement of the chamber is within 100 mm. The miniaturization of the oscillating thermocycler can be expected.

In our oscillatory device, the denaturation zone located between the annealing and extension zones is suggested as the appropriate arrangement of the heating blocks. Heat management without thermal cross-talk is easy. Finally, an improved oscillating device is demonstrated to execute the RT-PCR process directly, utilized to amplify the ca-nine distemper virus templates successfully, which could be well applied to a low-cost DNA analysis system in the future.