本研究成功製造了一套往復式熱循環儀，用於進行一步法逆轉錄聚合酶連鎖反應（RT-PCR）過程。該設備具有多項創新之處，包括將核糖核酸（RNA）混合物精準移液至鋁反應塊內的固定樣品體積，並通過線性運動控制系統，使樣品能夠在特定的RT-PCR加熱區域和自製的三組熱控制模塊之間移動。這樣的設計結合了腔體型和連續流型系統的優點，為PCR過程提供了更為精準和高效的環境。

在PCR過程中，反應室首先移動到低溫區域，完成RT階段並合成互補的脫氧核糖核酸（DNA）。隨後，低溫區域被調節到黏合區域，反應樣品在三個等溫區域之間來回移動，完成PCR過程，而無需額外的加熱區域。這樣的設計不僅節省了能源，還有效地降低了熱交叉的可能性。值得注意的是，反應室的移動位移總長度受限於100mm以內，這有助於確保PCR過程的穩定性和準確性。

最後，我們將自製的往復式設備應用於直接執行RT-PCR過程，並成功地用於放大犬瘟熱病毒模板。這一成就為低成本DNA分析系統的應用提供了新的可能性，同時也為未來相關研究提供了重要的技術基礎。

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The oscillating thermocycler is fabricated and performed in the present work to carry out the one-step RT-PCR process successfully.

 

The ribonucleic acid (RNA) mixture is pipetted into the fixed sample volume inside an aluminum reaction block. The sample oscillates the pathway onto the linear motion control system and through the specific RT-PCR heating zones with individual homemade thermal control modules.

 

The oscillating thermocycler combines the merits of the chamber type and the continuous-flow type systems.
 

Before PCR, the reaction chamber moves to the low-temperature zone to complete the RT stage and synthesize the complementary deoxyribonucleic acid (DNA). Next, the low-temperature zone is regulated to the annealing zone. Furthermore, the reactive sample is moved back and forth among three isothermal zones to complete PCR. No extra heating zone is required for the RT stage. The total length of the moving displacement of the chamber is within 100 mm. Our oscillatory device suggests the denaturation zone between the annealing and extension zones as the appropriate arrangement for the heating blocks. Heat management without thermal cross-talk is easy.
 

Finally, an improved oscillating device is demonstrated to execute the RT-PCR process directly and is utilized to amplify the canine distemper virus templates successfully, which could be well applied to a low-cost DNA analysis system.